Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was adult female ovaries of wild-type or transgenic fly line; bait produced from endogenous gene; prey produced from endogenous gene or transgenic construct.
The Cbl antibody was specific to the CblL (large) isoform.
