FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
smg, nanos
FlyBase ID
FBig0000108113
Interaction Network
Interactions Browser links
smg network
nanos network
Reported Interactions
FBrf0090804-1.XL.A
Reference
Smibert et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

nos

bait

[32]P label

nanos 
smg 

smaug

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
smaug recognition element
sufficient binding region
nt 21-80

coordinates relative to nos 3\' UTR

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type embryo extract.

(Smibert et al., 1996)
FBrf0123204-1.XL.A
Reference
Smibert et al., 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

nos

bait

[32]P label

nanos 
smg 

smaug

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
smaug recognition element
sufficient binding region
nt 21-80

coordinates relative to nos 3\' UTR

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as recombinant fusion protein in bacterial system.

(Smibert et al., 1999)
FBrf0183829-5.coIP.PCR
Reference
Semotok et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smg 

SMG

bait
nanos 

nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wild type embryos; bait produced from endogenous gene; prey produced from endogenous gene.

(Semotok et al., 2005)
FBrf0193114-2.coIP.PCR
Reference
Zaessinger et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smg 

Smg

bait
nanos 

nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males testes; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Zaessinger et al., 2006)
FBrf0212183-8.coIP.PCR
Reference
Rouget et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smg 

smg

bait
nanos 

nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Rouget et al., 2010)
FBrf0212721-2.PD.WB
Reference
Jeske et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 2embryonic stage 5embryonic stage 3embryonic stage 4embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

nos

bait

biotin label

nanos 
nanos 
smg 

Smaug

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
3 prime UTR
sufficient binding region
nt 7-161
nanos 
SRE
mutation disrupting interaction
nt 52-62
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced by in vitro translation.

(Jeske et al., 2011)

nos mRNA mising a 7-methylguanylate cap do not bind smg.

(Jeske et al., 2011)
FBrf0225982-7.coIP.PCR
Reference
Chen et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
smg 

smg

bait
nanos 

nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Chen et al., 2014)
FBrf0236765-1.PD.WB
Reference
Götze et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

nos

bait

biotin label

nanos 
nanos 
smg 

Smg

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
3-prime UTR
sufficient binding region
nt 7-161
nanos 
SRE
mutation disrupting interaction
CTGGC

both SREs in nos 3-prime UTR changed from CTGGC to CTCGC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type embryo extract.

(Götze et al., 2017)

Stoichiometry approximately 1:1 regardless of RNA length.

(Götze et al., 2017)
FBrf0236765-8.PD.MS
Reference
Götze et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
nanos 

nos

bait

biotin label

nanos 
nanos 
smg 

Smg

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nanos 
3-prime UTR
sufficient binding region
nt 7-161
nanos 
SRE
mutation disrupting interaction
CTGGC

both SREs in nos 3-prime UTR changed from CTGGC to CTCGC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type embryo extract.

(Götze et al., 2017)

Stoichiometry approximately 1:1 regardless of RNA length.

(Götze et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (8)