Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

Luciferase reporter assay.

bft miRNA was transfected into S2 cells.

Source was pupal eye discs of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Mutation of bft increases hid mRNA levels (but not pre-mRNA transcripts detected using intron-specific primers).