Addition of smo synthetic Smaug binding region peptide competed with smg-osk interaction, however mutated smo peptide containing L978E/L984E/L985 did not.

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

Fig5G

Source was S2R+ cells; bait and prey produced from transfected construct.

Bait protein was fused to ER membrane anchor. The ability of this chimera to recruit prey protein to the ER was taken as evidence of direct physical interaction.

Source was S2R+ cells; bait and prey produced from transfected construct.

Bait protein was fused to ER membrane anchor. The ability of this chimera to recruit prey protein to the ER was taken as evidence of direct physical interaction. osk-PC short isoform (139-

606) interacts with smg but does not interact with the PA isoform (long).

Split ubiquitin Y2H

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).