FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pall, RpS6
FlyBase ID
FBig0000108350
Interaction Network
Interactions Browser links
pall network
RpS6 network
Reported Interactions
FBrf0227703-1.coIP.MS
Reference
Xiao et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
pall 

PALL

bait

HA tag

RpS6 

RpS6

prey

11 unique peptides identified

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified RpS6 was phosphorylated at C-terminal Serine residues: S233, S235, S239, S242 and S245 (coordinates relative to RpS6-PB).

(Xiao et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Xiao et al., 2015)
FBrf0227703-2.coIP.WB
Reference
Xiao et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
RpS6 

RpS6

bait

FLAG tag or V5 tag

pall 

PALL

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The pall F-box domain is dispensable for the pall-RpS6 interaction.

(Xiao et al., 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xiao et al., 2015)
FBrf0227703-4.coIP.WB
Reference
Xiao et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
pall 

PALL

bait

HA tag

RpS6 

RpS6

prey

V5 tag

RpS6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
RpS6 
C-terminal phosphoserine residues
mutation disrupting interaction
aa 233-245

S233A, S235A, S239A, S242A and S245A, coordinates relative to RpS6-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Xiao et al., 2015)

The pall F-box domain is dispensable for the pall-RpS6 interaction.

(Xiao et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)