Dredd, Diap2 Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Diap2

bait

HA tag

Dredd

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Guntermann and Foley, 2011)

FBrf0218688-1.PD.WB

Reference

Meinander et al., 2012

Description

physical association

Assay

pull down

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Diap2

bait

GST tag

Diap2

Dredd

prey

V5 tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Diap2

BIR domains

sufficient binding region

aa 1-338

Diap2

BIR domains

necessary binding region

aa 1-280

Diap2

BIR2 or BIR3

sufficient binding region

unspecified

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Meinander et al., 2012)

FBrf0218688-2.coIP.WB

Reference

Meinander et al., 2012

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Dredd

bait

V5 or HA tag

Diap2

prey

HA, V5 or GST tag

Diap2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Diap2

BIR domains

sufficient binding region

aa 1-338

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Meinander et al., 2012)

FBrf0218688-3.coIP.WB

Reference

Meinander et al., 2012

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Diap2

bait

HA tag

Dredd

prey

V5 tag

Dredd

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Dredd

prodomain DED1

sufficient binding region

aa 1-142

Dredd

prodomain DED1

mutation decreasing interaction

aa 1-142

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Meinander et al., 2012)

FBrf0229433-1.coIP.WB

Reference

Guntermann et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Diap2

IAP2

bait

HA tag

Diap2

Dredd

prey

Myc tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Diap2

central region

sufficient binding region

aa 180-450

coordinates relative to Diap2-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Guntermann et al., 2015)

FBrf0229433-2.coIP.WB

Reference

Guntermann et al., 2015

Description

physical association

Assay

Collection

Source/Stage

Cell line used