Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Deletion analysis suggests two non-overlapping binding domains as two adjacent regions are sufficient for interaction.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from transfected construct.

Interaction enhanced by PKA/CK1-mediated phosphorylation.

Source was live S2 cells; proteins produced from transfected constructs.

Treating cells with hh increases signal.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The smo-Gprk2 interaction increases upon treatment with hh or PI(4)P.