FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
wts, Zyx
FlyBase ID
FBig0000108426
Interaction Network
Interactions Browser links
wts network
Zyx network
Reported Interactions
FBrf0213900-3.coIP.WB
Reference
Rauskolb et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Zyx 

Zyx

bait

V5 tag

Zyx 
wts 

wts

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Zyx 
LIM domains
sufficient binding region
aa 386-585

coordinates relative to Zyx-PB, full-length Zyx does not interact with wts.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rauskolb et al., 2011)

Zyx, d, and wts thought to form trimeric complex. Interactions captured pairwise.

(Rauskolb et al., 2011)
FBrf0213900-4.coIP.WB
Reference
Rauskolb et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
wts 

wts

bait

FLAG tag

wts 
wts 
Zyx 

Zyx

prey

V5 tag

Zyx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Zyx 
LIM domains
sufficient binding region
aa 386-585

coordinates relative to Zyx-PB, full-length Zyx does not interact with wts.

wts 
interaction domain
sufficient binding region
aa 1-708
wts 
interaction domain
necessary binding region
aa 192-708
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Rauskolb et al., 2011)

Zyx, d, and wts thought to form trimeric complex. Interactions captured pairwise.

(Rauskolb et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)