FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ed, sav
FlyBase ID
FBig0000108443
Interaction Network
Interactions Browser links
ed network
sav network
Reported Interactions
FBrf0217474-1.coIP.WB
Reference
Yue et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ed 

Ed

bait

MYC tag

ed 
sav 

Sav

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ed 
C terminal domain
necessary binding region
aa 1018-1332

coordinates relative to ed-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yue et al., 2012)
FBrf0217474-2.coIP.WB
Reference
Yue et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
sav 

Sav

bait

HA tag

sav 
sav 
ed 

Ed

prey

MYC tag

ed 
ed 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ed 
intracellular domain
sufficient binding region
aa 996-1332

coordinates relative to ed-PA

ed 
intracellular domain
necessary binding region
aa 1115-1332

coordinates relative to ed-PA

sav 
WW domain
sufficient binding region
sav 
N terminal region
necessary binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yue et al., 2012)
FBrf0217474-9.FRET.FM
Reference
Yue et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ed 

Ed

fluorescence donor

CFP tag

ed 

Ed

fluorescence donor

CFP tag

ed 

Ed

fluorescence donor

CFP tag

sav 

Sav

fluorescence acceptor

YFP tag

sav 

Sav

fluorescence acceptor

YFP tag

sav 

Sav

fluorescence acceptor

YFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Yue et al., 2012)

N terminally CFP tagged ed showed the strongest interaction with C terminally YFP tagged sav in this experiment.

(Yue et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)