FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
nej, EcR
FlyBase ID
FBig0000108460
Interaction Network
Interactions Browser links
nej network
EcR network
Reported Interactions
FBrf0216319-1.coIP.WB
Reference
Kirilly et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
nej 

nej

bait

HA tag

nej 
EcR 

EcR

prey

FLAG or Myc tag

EcR 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
nej 
interaction domain
sufficient binding region
aa 1-1506
EcR 
interaction domain
mutation decreasing interaction
aa 655-878, aa 650

deletion of aa 655-878 and W650A, coordinates relative to EcR-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kirilly et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)