FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
gro
FlyBase ID
FBig0000108476
Interaction Network
Interactions Browser links
gro network
Reported Interactions
FBrf0174967-1.CH.A
Reference
Song et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

GroN

bait

His tag

gro 

Gro

prey
gro 
gro 
gro 
gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
N-terminal domain
sufficient binding region
aa 1-194

coordinates relative to gro-PA

gro 
coiled-coil domain residue 38
mutation decreasing interaction
aa 38

L38D, coordinates relative to gro-PA

gro 
coiled-coil domain residue 87
mutation decreasing interaction
aa 87

L87D, coordinates relative to gro-PA

gro 
coiled-coil domain residues 38,87
mutation disrupting interaction
aa 38,87

L38D,L87D, coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced and labeled by in vitro translation.

(Song et al., 2004)
FBrf0174967-2.CH.MW
Reference
Song et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
gro 

Gro

neutral component

FLAG tag

gro 
gro 

Gro

neutral component

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
coiled-coil domain residues 38,87
mutation disrupting interaction
aa 38,87

L38D,L87D, coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein immunopurified from transfected S2 cell nuclear extract.

(Song et al., 2004)

The L38D,L87D mutant form of gro elutes as a monomer instead of the tetramer expected of wildtype gro.

(Song et al., 2004)
FBrf0174967-3.coIP.WB
Reference
Song et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
gro 

Gro

bait

GAL4 tag

gro 

Gro

prey
gro 
gro 
gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
coiled-coil domain residue 38
mutation decreasing interaction
aa 38

L38P, coordinates relative to gro-PA

gro 
coiled-coil domain residue 87
mutation decreasing interaction
aa 87

L87P, coordinates relative to gro-PA

gro 
coiled-coil domain residues 38,87
mutation disrupting interaction
aa 38,87

L38P,L87P, coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Song et al., 2004)
FBrf0174967-4.coIP.WB
Reference
Song et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

Gro

bait

Myc tag

gro 

Gro

prey
gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
coiled-coil domain residues 38,87
mutation disrupting interaction
aa 38,87

L38D,L87D, coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Song et al., 2004)
FBrf0200724-1a.BN.A
Reference
Jennings et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

gro

self

[35]S label

gro 

gro

self

[35]S label

gro 
gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
interaction domain
necessary binding region
aa 1-58

coordinates relative to gro-PA

gro 
interaction domain
mutation disrupting interaction
aa 38,87

L38P, L87P, coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced and labeled by in vitro translation.

(Jennings et al., 2008)
FBrf0200724-2a.BN.WB
Reference
Jennings et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

gro

self
gro 

gro

self
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; proteins produced from endogenous gene.

(Jennings et al., 2008)
FBrf0200724-3.PD.A
Reference
Jennings et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

gro

bait

GST tag

gro 

gro

prey

[35]S label

gro 
gro 
gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
interaction domain
necessary binding region
aa 1-58

coordinates relative to gro-PA

gro 
interaction domain
mutation disrupting interaction
aa 38,87

L38P, L87P, coordinates relative to gro-PA

gro 
interaction domain
mutation decreasing interaction
aa 19-27

coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Jennings et al., 2008)
FBrf0206310-2.PD.A
Reference
Mieszczanek et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

Gro

bait

GST tag

gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Mieszczanek et al., 2008)
FBrf0212487-1.CH.MW
Reference
Kuo et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

GroQ

self

SUMO, His tag

gro 

GroQ

self

SUMO, His tag

gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
Q domain
sufficient binding region
aa 1-133

coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Kuo et al., 2011)

gro Q domain forms oligomers.

(Kuo et al., 2011)
FBrf0212487-2.CS.MW
Reference
Kuo et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

GroQ

self

SUMO, His tag

gro 

GroQ

self

SUMO, His tag

gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
Q domain
sufficient binding region
aa 1-133

coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

(Kuo et al., 2011)

Sedimentation data consistent with a mixture of monomer, tetramer and 16-mer.

(Kuo et al., 2011)
FBrf0217447-1.PD.A
Reference
Turki-Judeh and Courey, 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gro 

Gro

bait

His tag, [35]S label

gro 

Gro

prey

[35]S label

gro 
gro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gro 
N-terminal domain
sufficient binding region
aa 1-194

coordinates relative to gro-PA

gro 
N-terminal domain
mutation disrupting interaction
aa 38,87

L38D,L87D, coordinates relative to gro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Turki-Judeh and Courey, 2012)
External Crossreferences and Linkouts ( 0 )
References (5)