FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
papi, AGO3
FlyBase ID
FBig0000108477
Interaction Network
Interactions Browser links
papi network
AGO3 network
Reported Interactions
FBrf0213491-14.coIP.WB
Reference
Liu et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
papi 

PAPI

bait
AGO3 

AGO3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Liu et al., 2011)
FBrf0213491-3.Y2H
Reference
Liu et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO3 

AGO3

bait

fused to LexA DNA-binding domain

AGO3 
AGO3 
papi 

PAPI

prey

fused to B42 activation domain

papi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
papi 
C-terminal region
sufficient binding region
aa 257-576

coordinates relative to papi-PA

AGO3 
N-terminal region
sufficient binding region
aa 1-289

coordinates relative to AGO3-PD

AGO3 
symmetric dimethylation sites R68-R72
sufficient binding region
aa 68-72

"MUT" construct (R68K + R70K + R72K), coordinates relative to AGO3-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Liu et al., 2011)

Two-hybrid system: yeast LexA-BD/B42-AD

(Liu et al., 2011)
FBrf0213491-5.coIP.WB
Reference
Liu et al., 2011
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
papi 

PAPI

bait
AGO3 

AGO3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Liu et al., 2011)

The papi-AGO3 interaction is RNA-independent (unaffected by RNase treatment).

(Liu et al., 2011)
FBrf0213491-6.coIP.WB
Reference
Liu et al., 2011
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
AGO3 

AGO3

bait
papi 

PAPI

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Liu et al., 2011)

The papi-AGO3 interaction is RNA-independent (unaffected by RNase treatment).

(Liu et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)