FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Polr2A, HP1c
FlyBase ID
FBig0000108488
Interaction Network
Interactions Browser links
Polr2A network
HP1c network
Reported Interactions
FBrf0212000-16.coIP.WB
Reference
Kwon et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
HP1c 

HP1c

bait

FLAG tag

Polr2A 

RpII215

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Kwon et al., 2010)
FBrf0212000-17.PD.WB
Reference
Kwon et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Polr2A 

RpII215

bait

biotinylated

HP1c 

HP1c

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; recombinant proteins produced in baculovirus and insect cell system.

(Kwon et al., 2010)
FBrf0212000-18.PD.WB
Reference
Kwon et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Polr2A 

RpII215

bait

biotinylated

HP1c 

HP1c

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; recombinant proteins produced in baculovirus and insect cell system.

(Kwon et al., 2010)
FBrf0241416-38.coIP.WB
Reference
Lee et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
HP1c 

HP1c

bait

FLAG tag

Polr2A 

PolII

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct or endogenous gene; prey produced from endogenous gene.

(Lee et al., 2019)
FBrf0241416-41.PD.WB
Reference
Lee et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Polr2A 

PolII

bait

biotin label

HP1c 

HP1c

prey

FLAG tag

HP1c 
HP1c 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
HP1c 
Chromo domain
sufficient binding region
aa 1-78
HP1c 
Chromo domain
necessary binding region
aa 1-60
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptide chemically synthesized and labeled in vitro; prey produced as a recombinant fusion protein in baculovirus and Sf21 cell system.

(Lee et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)