FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
gcm, Su(var)2-10
FlyBase ID
FBig0000108491
Interaction Network
Interactions Browser links
gcm network
Su(var)2-10 network
Reported Interactions
FBrf0208351-13.coIP.WB
Reference
Jacques et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
gcm 

Gcm

bait

FLAG tag

Su(var)2-10 

dpias

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jacques et al., 2009)
FBrf0208351-8.Y2H
Reference
Jacques et al., 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gcm 

gcm

bait

fused to LexA DNA-binding domain

gcm 
Su(var)2-10 

dpias

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gcm 
N-terminal region
sufficient binding region
aa 1-261

The N-terminal construct of gcm used as bait includes the DBD, the NLS and the PEST domain.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Jacques et al., 2009)

Two-hybrid system: yeast LexA-BD/VP16-AD

(Jacques et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)