FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
rl, krz
FlyBase ID
FBig0000108511
Interaction Network
Interactions Browser links
rl network
krz network
Reported Interactions
FBrf0211995-1.coIP.WB
Reference
Tipping et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
rl 

rl

bait

FLAG tag

krz 

krz

prey

HA or Myc tag

krz 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
krz 
region residue 209
mutation increasing interaction
aa 209

R209E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction decreased by coexpression of Dsor1

(Tipping et al., 2010)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tipping et al., 2010)
FBrf0211995-2.coIP.WB
Reference
Tipping et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
krz 

krz

bait

HA tag

krz 
rl 

rl

prey

FLAG tag

rl 
rl 
rl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
krz 
region residue 209
mutation increasing interaction
aa 209

R209E

rl 
TEY motif residue 198
mutation decreasing interaction
aa 198

T198E, T198A

rl 
TEY motif residue 200
mutation decreasing interaction
aa 200

Y198E, Y200F

rl 
region residue 334
mutation decreasing interaction
aa 334

D334N

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tipping et al., 2010)

Interaction not detected by western blot with antidpERK antibody suggesting krz preferentially binds to inactive/unphosphorylated rl.

(Tipping et al., 2010)
FBrf0211995-4.coIP.WB
Reference
Tipping et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
krz 

krz

bait

HA tag

rl 

rl

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced by in vitro translation

(Tipping et al., 2010)

krzR209E used.

(Tipping et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)