Interaction in vitro; enzyme produced as a recombinant fusion protein in bacterial system.

Elp3 autoacetylates itself.

Elp3 was incubated with acetyl-coA and reaction products were detected using atibodies to acetylated lysine.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Elp1 through Elp6 plus Hsc70-4 comprise the Elongator Complex.