FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ed, cno
FlyBase ID
FBig0000108592
Interaction Network
Interactions Browser links
ed network
cno network
Reported Interactions
FBrf0187414-6.coIP.WB
Reference
Wei et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ed 

Ed

bait
cno 

Cno

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wei et al., 2005)
FBrf0187414-7.PD.A
Reference
Wei et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ed 

Ed

bait

GST tag

ed 
ed 
cno 

Cno

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ed 
C-terminal region
sufficient binding region
aa 1283-1332

coordinates relative to ed-PA

ed 
C-terminal EIIV motif
necessary binding region
EIIV
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Wei et al., 2005)
FBrf0251832-1.XC
Reference
Perez-Vale et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cno 

cno

neutral component

His tag

cno 

cno

neutral component

His tag

cno 
cno 

cno

neutral component

His tag

cno 
ed 

ed

neutral component

His tag

ed 
ed 

ed

neutral component

His tag

ed 

ed

neutral component

His tag

ed 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cno 
PDZ domain residues 1006-1099
sufficient binding region
aa 1006-1099
ed 
C terminal tail residues 1008-1099
sufficient binding region
aa 1008-1099
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The PDZ domain of cno and the c terminal tail of ed were fused into one construct. The crystal structure demonstrated which residues interact.

(Perez-Vale et al., 2021)

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Perez-Vale et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)