FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Dronc, numb
FlyBase ID
FBig0000108599
Interaction Network
Interactions Browser links
Dronc network
numb network
Reported Interactions
FBrf0213702-1.coIP.WB
Reference
Ouyang et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

numb

bait

Myc tag

numb 
numb 
Dronc 

Dronc

prey

FLAG tag

Dronc 
Dronc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
phospho-tyrosine binding domain
sufficient binding region
aa 78-204

coordinates relative to numb-PA

numb 
region residues 143,183,188,248,447
mutation disrupting interaction
aa 143,183,188,248,447

T143D,S183D,S188D,S248D,S447D, coordinates relative to numb-PA

Dronc 
interaction domain
sufficient binding region
aa 251-450
Dronc 
interaction domain
necessary binding region
aa 352-450
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ouyang et al., 2011)
FBrf0213702-2.coIP.WB
Reference
Ouyang et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Dronc 

Dronc

bait

FLAG tag

Dronc 
numb 

numb

prey

Myc tag

numb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
numb 
phospho-tyrosine binding domain
necessary binding region
aa 78-204

coordinates relative to numb-PA

Dronc 
interaction domain
sufficient binding region
aa 135-450
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ouyang et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)