Source was mammalian cell line HEK293; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Interaction observed in presence of EcR. Interaction increased upon treatment of cells with ecdysone agonist.

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

smt3 biotinylated in vivo by coexpression of bacterial BirA enzyme. Proteins purified under denaturing conditions to inactivate enzymes and dissociate smt3-binding proteins, leaving only covalently bound proteins.