FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Nlp
FlyBase ID
FBig0000108616
Interaction Network
Interactions Browser links
Nlp network
Reported Interactions
FBrf0093082-1.XL.MW
Reference
Crevel et al., 1997
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlp 

CRP1

self
Nlp 

CRP1

self
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein purified from embryonic extract.

(Crevel et al., 1997)

Crosslinking of purified Nlp protein and resolution by SDS-PAGE indicates that Nlp may form homotetrameric or homopentameric complexes.

(Crevel et al., 1997)
FBrf0093082-2.EM
Reference
Crevel et al., 1997
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlp 

CRP1

self
Nlp 

CRP1

self
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein purified from embryonic extract.

(Crevel et al., 1997)

Nlp is observed to form homotetramers, although some particles appeared to be pentameric.

(Crevel et al., 1997)
FBrf0240882-1.coIP.WB
Reference
Anselm et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Nlp 

NLP

bait

V5 tag

Nlp 

NLP

prey

HA tag

Nlp 
Nlp 
Nlp 
Nlp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nlp 
interaction domain residue 76
mutation decreasing interaction
aa 76

N76R, coordinates relative to Nlp-PA

Nlp 
interaction domain residue 6
mutation disrupting interaction
aa 6

F6E, coordinates relative to Nlp-PA

Nlp 
interaction domain residue 62
mutation decreasing interaction
aa 62

I62E, coordinates relative to Nlp-PA

Nlp 
interaction domain residue 79
mutation disrupting interaction
aa 79

V79E, coordinates relative to Nlp-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Anselm et al., 2018)
FBrf0240882-6.CM.MW
Reference
Anselm et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nlp 

NLP

neutral component
Nlp 

NLP

neutral component
Nlp 
Nlp 
Nlp 
Nlp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nlp 
core domain
sufficient binding region
unspecified
Nlp 
interaction domain residue 6
mutation disrupting interaction
aa 6

F6E, coordinates relative to Nlp-PA

Nlp 
interaction domain residue 62
mutation disrupting interaction
aa 62

I62E, coordinates relative to Nlp-PA

Nlp 
interaction domain residue 79
mutation disrupting interaction
aa 79

V79E, coordinates relative to Nlp-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Anselm et al., 2018)
External Crossreferences and Linkouts ( 0 )
References (2)