Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Uses sevS11 allele, a constitutively activated form that consists of the C-terminal Sev protein, which includes the intracellular tyrosine kinase domain, bound to the signal peptide of the Drosophila cuticle protein CP3 and the 10 amino acid Myc epitope.
Interaction only detected with version of Socs36E consisting of aa 414-632, not with full length version.
Source was live S2 cells; proteins produced from transfected constructs.
Uses sevS11 allele, a constitutively activated form that consists of the C-terminal Sev protein, which includes the intracellular tyrosine kinase domain, bound to the signal peptide of the Drosophila cuticle protein CP3 and the 10 amino acid Myc epitope.
