FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
papi, aub
FlyBase ID
FBig0000108708
Interaction Network
Interactions Browser links
papi network
aub network
Reported Interactions
FBrf0213491-13.coIP.WB
Reference
Liu et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
papi 

PAPI

bait
aub 

AUB

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Liu et al., 2011)
FBrf0213491-4.Y2H
Reference
Liu et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aub 

AUB

bait

fused to LexA DNA-binding domain

papi 

PAPI

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Liu et al., 2011)

Weak interaction.

(Liu et al., 2011)

Two-hybrid system: yeast LexA-BD/B42-AD

(Liu et al., 2011)
FBrf0238516-9.ITC
Reference
Zhang et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
papi 

Papi

bait
papi 
aub 

Aub

prey
aub 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
papi 
eTud domain
sufficient binding region
aa 259-479

coordinates relative to papi-PA

aub 
(G,A)R repeat
sufficient binding region
aa 9-18aa 81-90

Kd=157uM for unmethylated aub

Kd=218uM for unmethylated aub

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptides were commercially synthesized.

(Zhang et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)