FB2025_04 , released October 2, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
brat, eIF4EHP
FlyBase ID
FBig0000108750
Interaction Network
Interactions Browser links
brat network
eIF4EHP network
Reported Interactions
FBrf0193740-1.coIP.WB
Reference
Cho et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4EHP 

d4EHP

bait
brat 

Brat

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Cho et al., 2006)
FBrf0193740-2.coIP.WB
Reference
Cho et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brat 

Brat

bait

FLAG tag

brat 
brat 
brat 
brat 
brat 
brat 
eIF4EHP 

d4EHP

prey

HA tag

eIF4EHP 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
brat 
NHL domain
sufficient binding region

coordinates not reported

brat 
NHL domain
necessary binding region

coordinates not reported

brat 
NHL domain residue K882
mutation disrupting interaction
aa 882

K882E, coordinates relative to brat-PA

brat 
NHL domain residue R837
mutation disrupting interaction
aa 837

R837D, coordinates relative to brat-PA

brat 
NHL domain residues K809 and E810
mutation decreasing interaction
aa 809-810

K809A and E810A, coordinates relative to brat-PA

brat 
NHL domain residue G860
mutation decreasing interaction
aa 860

G860D, coordinates relative to brat-PA

eIF4EHP 
third dorsal alpha-helix
necessary binding region
aa 179-194

coordinates relative to eIF4EHP-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Cho et al., 2006)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)