Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/B42-AD
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
