FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
put, sax
FlyBase ID
FBig0000108817
Interaction Network
Interactions Browser links
put network
sax network
Reported Interactions
FBrf0080209-3.coIP.XL.A
Reference
Letsou et al., 1995
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sax 

sax

bait

HA tag, [125]I label

put 

Punt

prey

His tag, [125]I label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS cell line; bait produced from transfected construct; prey produced from transfected construct.

(Letsou et al., 1995)

When co-transfected, sax and put form a heteromeric complex with [125]I-labeled BMP2. Alone, neither sax nor put binds BMP2.

(Letsou et al., 1995)

Cells were transfected with sax and put then incubated with [125]I-labeled human BMP2 and crosslinked by disuccinimidyl suberate. Proteins binding to BMP2 are radiolabeled in SDS-PAGE.

(Letsou et al., 1995)
FBrf0080209-4.coIP.XL.A
Reference
Letsou et al., 1995
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
put 

Punt

bait

His tag, [125]I label

sax 

sax

prey

HA tag, [125]I label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of COS cell line; bait produced from transfected construct; prey produced from transfected construct.

(Letsou et al., 1995)

When co-transfected, sax and put form a heteromeric complex with [125]I-labeled BMP2. Alone, neither sax nor put binds BMP2.

(Letsou et al., 1995)

Cells were transfected with sax and put then incubated with [125]I-labeled human BMP2 and crosslinked by disuccinimidyl suberate. Proteins binding to BMP2 are radiolabeled in SDS-PAGE.

(Letsou et al., 1995)
FBrf0211154-13.coIP.WB
Reference
Haerry, 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
put 

PUT

bait

FLAG tag

sax 

SAX

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Haerry, 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)