FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Imp, upd1
FlyBase ID
FBig0000108819
Interaction Network
Interactions Browser links
Imp network
upd1 network
Reported Interactions
FBrf0218483-1.coIP.PCR
Reference
Toledano et al., 2012
Description
physical association
Assay
Collection
Source/Stage
testis / maleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Imp 

Imp

bait

GFP tag

upd1 

upd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult males testes; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Toledano et al., 2012)
FBrf0218483-2.PD.WB
Reference
Toledano et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
upd1 

upd

bait

biotin label

upd1 
Imp 

Imp

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
upd1 
3-prime UTR
sufficient binding region
nt 4-243
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced by in vitro translation.

(Toledano et al., 2012)
FBrf0241537-2.coIP.PCR
Reference
Sreejith et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Imp 

Imp

bait

HA tag

upd1 

upd

prey
upd1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
upd1 
3-prime UTR
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Sreejith et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)