FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eIF4E1, 4E-T
FlyBase ID
FBig0000108898
Interaction Network
Interactions Browser links
eIF4E1 network
4E-T network
Reported Interactions
FBrf0226072-11.PD.MW
Reference
Igreja et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
4E-T 

4E-T

bait

MBP tag

4E-T 
eIF4E1 

eIF4E

prey

His tag

eIF4E1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E1 
C-terminal region
sufficient binding region
aa 69-248

coordinates relative to eIF4E1-PC

4E-T 
eIF4E-binding region
sufficient binding region
aa 9-44

coordinates relative to 4E-T-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Igreja et al., 2014)
FBrf0226072-16.ITC
Reference
Igreja et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait

His tag

eIF4E1 
4E-T 

4E-T

prey

GB1 tag

4E-T 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E1 
C-terminal region
sufficient binding region
aa 69-248

coordinates relative to eIF4E1-PC

4E-T 
eIF4E-binding region
sufficient binding region
aa 9-44

coordinates relative to 4E-T-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Igreja et al., 2014)

Kd = 5.6 nM

(Igreja et al., 2014)
FBrf0226072-4.coIP.WB
Reference
Igreja et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait

HA tag

eIF4E1 
eIF4E1 
4E-T 

4E-T

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E1 
dorsal binding surface residue W106
mutation disrupting interaction
aa 106

W106A, coordinates relative to eIF4E1-PC

eIF4E1 
lateral binding surface residues I96 and I112
mutation disrupting interaction
aa 96-112

"II-AA" construct (I96A + I112A), coordinates relative to eIF4E1-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Igreja et al., 2014)

Mutation of residues on the lateral surface of eIF4E1 (I96A and I112A) specifically disrupts interaction with 4E-BP-type proteins (Thor, cup and 4E-T) but does not affect binding to eIF4G1.

(Igreja et al., 2014)
FBrf0226072-8.coIP.WB
Reference
Igreja et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
4E-T 

4E-T

bait

HA tag

4E-T 
4E-T 
4E-T 
4E-T 
4E-T 
eIF4E1 

eIF4E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
4E-T 
N-terminal region
sufficient binding region
aa 1-511

coordinates relative to 4E-T-PB

4E-T 
canonical 4E-binding motif residues Y10 and L15
mutation disrupting interaction
aa 10-15

"C*" construct (Y10A + L15A), coordinates relative to 4E-T-PB

4E-T 
non-canonical 4E-binding motif
necessary binding region
aa 28-43

"ΔNC" construct, coordinates relative to 4E-T-PB

4E-T 
non-canonical 4E-binding motif residues L36 and L39
mutation disrupting interaction
aa 36-39

"NC*" construct (L36A + L39A), coordinates relative to 4E-T-PB

4E-T 
non-canonical 4E-binding motif residues F41-K43
mutation disrupting interaction
aa 41-43

"FWK-3xA" construct (F41A + W42A + K43A), coordinates relative to 4E-T-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Igreja et al., 2014)
FBrf0243046-14.coIP.WB
Reference
Ruscica et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

bait

HA tag

4E-T 

4E-T

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ruscica et al., 2019)
FBrf0250268-2.XC
Reference
Peter et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4E1 

eIF4E

neutral component
eIF4E1 
eIF4E1 

eIF4E

neutral component
eIF4E1 
eIF4E1 

eIF4E

neutral component
4E-T 
4E-T 

4E-T

neutral component
4E-T 

4E-T

neutral component
4E-T 
4E-T 

4E-T

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4E1 
interaction domain
sufficient binding region
aa 1-248

coordinates relative to eIF4E1-PC

4E-T 
interaction domain
sufficient binding region
aa 9-44
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Peter et al., 2015)
FBrf0250268-5.PD.MW
Reference
Peter et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
4E-T 

4E-T

bait

MBP tag

4E-T 
4E-T 
eIF4E1 

eIF4E

prey

Strep-His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
4E-T 
canonical and non-canonical domains
mutation disrupting interaction
aa 10, 15, 41 and 42

Y10A, L15A, F41D, W42D; within the context of aa 9-44

4E-T 
structurally conserved residues
mutation disrupting interaction
aa 12, 19, 43

K12E, R19E, K43E, W42D; within the context of aa 9-44.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.

(Peter et al., 2015)
FBrf0250268-8.coIP.WB
Reference
Peter et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
4E-T 

4E-T

bait

GFP tag

4E-T 
4E-T 
eIF4E1 

eIF4E

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
4E-T 
canonical domain residues 10 and 15
mutation disrupting interaction
aa 10 and 15

Y10A, L15A, within the context of aa 9-44.

4E-T 
non-canonical domain residues 41 and 42
mutation disrupting interaction
aa 41 and 42

F41D, W42D, within the context of aa 9-44.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Peter et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)