Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Embryos were formaldehyde crosslinked before affinity purification.
Interaction in vitro; bait derived from tagged transgenic construct and purified from ovaries; prey produced as a recombinant fusion protein in baculovirus and Sf9 cell system.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.