cv-c also interacts weakly with Cdc42 in a NaF-specific manner.

cv-c specifically coimmunoprecipitates Rho1 in the presence of NaF, but not in its absence. This suggests that cv-c has specific GAP activity for Rho1.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The specificity of cv-c binding to various GTPases was determined with a biochemical trapping assay that uses NaF to stabilize GTPases in their GTPase transition state.

Positive control.