Interaction in CHO cell system; ligand derived from wild-type larval extract; receptor produced from transfected construct.

HPLC fractions tested for ability to activate AkhR in a heterologous CHO cell system. Protein in HPLC fractions was identified by mass spectrometry.

Source was frog oocytes; bait produced from injected RNA construct; prey peptide was synthesized in vitro.

Interaction inferred from inward currents across the plasma membrane in response to peptide application, measured by voltage clamp.

Interaction in CHO cell system; ligand synthesized in vitro; receptor produced from transfected construct.

Interaction based on ability of Akh to induce increase in [Ca2+] through AkhR in a heterologous system. Interaction inferred.