Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.
D forms a complex with ftz-f1 on the ftz zebra regulatory element. D does not bind to the ftz regulatory element alone. The zebra element was gel shifted after incubation with ftz-f1 alone. In the presence of D and ftz-f1, a supershifted complex formed. Antibodies specific either ftz-f1 or D further shifted the complex.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.