Source was adult male testes of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Q70L, constitutively active form, coordinates relative to Rab1-PA
S25N, dominant negative form, coordinates relative to Rab
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/B42-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was adult male spermatocytes of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction decreased in a Cog7 mutant background.