FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000109190
Interaction Network
Interactions Browser links
Rab1 network
sau network
Reported Interactions
FBrf0234570-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sau 

GOLPH3

bait
Rab1 

Rab1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult male testes of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0234570-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sau 

GOLPH3

bait

fused to LexA DNA-binding domain

Rab1 

Rab1

prey

fused to B42 activation domain

Rab1 
Rab1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab1 
residue Q70
mutation increasing interaction
aa 70

Q70L, constitutively active form, coordinates relative to Rab1-PA

Rab1 
residue S25
mutation decreasing interaction
aa 25

S25N, dominant negative form, coordinates relative to Rab

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD

FBrf0234570-4.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab1 

Rab1

bait

GST tag

sau 

GOLPH3

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

sau binds more strongly to Rab1 in the presence of GTP than GDP.

FBrf0234570-7.PLA.FM
Description
physical association
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sau 

GOLPH3

neutral component
sau 

GOLPH3

neutral component
sau 

GOLPH3

neutral component
Rab1 

Rab1

neutral component
Rab1 

Rab1

neutral component
Rab1 

Rab1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult male spermatocytes of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

FBrf0237100-9.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sau 

GOLPH3

bait
Rab1 

Rab1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Interaction decreased in a Cog7 mutant background.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)