FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mod(mdg4), Chro
FlyBase ID
FBig0000109210
Interaction Network
Interactions Browser links
mod(mdg4) network
Chro network
Reported Interactions
FBrf0224369-1.coIP.WB
Reference
Golovnin et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

mod(mdg4)

bait
mod(mdg4) 
Chro 

Chro

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Golovnin et al., 2014)
FBrf0224369-2.coIP.WB
Reference
Golovnin et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Chro 

Chro

bait
mod(mdg4) 

mod(mdg4)

prey
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Golovnin et al., 2014)
FBrf0224369-3.Y2H
Reference
Golovnin et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chro 

Chro

bait

fused to GAL4 DNA-binding domain

mod(mdg4) 

mod(mdg4)

prey

fused to GAL4 activation domain

mod(mdg4) 
mod(mdg4) 
mod(mdg4) 
mod(mdg4) 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mod(mdg4) 
N-terminal BTB-Q domains
sufficient binding region
unspecified
mod(mdg4) 
N-terminal BTB domain
necessary binding region
unspecified
mod(mdg4) 
N-terminal Q domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Golovnin et al., 2014)
FBrf0224369-4.Y2H
Reference
Golovnin et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mod(mdg4) 

mod(mdg4)

bait

fused to GAL4 DNA-binding domain

mod(mdg4) 
Chro 

Chro

prey

fused to GAL4 activation domain

Chro 
Chro 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Chro 
C-terminal domain
sufficient binding region
unspecified
Chro 
C-terminal domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Golovnin et al., 2014)
FBrf0253430-23.PD.MS
Reference
Kaushal et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chro 

Chro

bait

GFP tag

Chro 
mod(mdg4) 

mod(mdg4)

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Chro 
C terminal domain
sufficient binding region
aa 613-926

coordinates relative to Chro-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type, nuclear embryonic extract.

(Kaushal et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)