His2A, Jabba Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

His2A, Jabba

FlyBase ID

FBig0000109345

Interaction Network

Interactions Browser links

His2A network
Jabba network

Reported Interactions

FBrf0234959-37.BiLC.LUC

Reference

Kolkhof et al., 2017

Description

physical association

Assay

split luciferase complementation

luminiscence technology

Collection

Source/Stage

Cell line used

Kc167

Participants

Corresponds to

Reported as

Role

Note

Jabba

neutral component

Gaussia luciferase tag, N-terminal fragment

Jabba

neutral component

Gaussia luciferase tag, N-terminal fragment

Jabba

neutral component

Gaussia luciferase tag, N-terminal fragment

His2A

H2A

neutral component

Gaussia luciferase tag, C-terminal fragment

His2A

H2A

neutral component

Gaussia luciferase tag, C-terminal fragment

His2A

H2A

neutral component

Gaussia luciferase tag, C-terminal fragment

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Strong interaction.

(Kolkhof et al., 2017)

Source was Kc167 cells; proteins produced from transfected constructs.

(Kolkhof et al., 2017)

Cells treated with 400uM oleic acid to induce lipid droplet formation in cells.

(Kolkhof et al., 2017)

FBrf0234959-44.BiLC.LUC

Reference

Kolkhof et al., 2017

Description

physical association

Assay

split luciferase complementation

luminiscence technology

Collection

Source/Stage

Cell line used

Kc167

Participants

Corresponds to

Reported as

Role

Note

His2A

H2A

neutral component

Gaussia luciferase tag, N-terminal fragment

His2A

H2A

neutral component

Gaussia luciferase tag, N-terminal fragment

His2A

H2A

neutral component

Gaussia luciferase tag, N-terminal fragment

Jabba

neutral component

Gaussia luciferase tag, C-terminal fragment

Jabba

neutral component

Gaussia luciferase tag, C-terminal fragment

Jabba

neutral component

Gaussia luciferase tag, C-terminal fragment

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Strong interaction.

(Kolkhof et al., 2017)

Source was Kc167 cells; proteins produced from transfected constructs.

(Kolkhof et al., 2017)

Cells treated with 400uM oleic acid to induce lipid droplet formation in cells.

(Kolkhof et al., 2017)

FBrf0249777-2.BiLC.LUC

Reference

Stephenson et al., 2021

Description

physical association

Assay

split luciferase complementation

luminiscence technology

Collection

Source/Stage

Cell line used

Kc167

Participants

Corresponds to

Reported as

Role

Note

Jabba

unspecified role

Gaussia luciferase tag, N or C terminal fragment

Jabba

unspecified role

Gaussia luciferase tag, N or C terminal fragment

Jabba

unspecified role

Gaussia luciferase tag, N or C terminal fragment

Jabba

His2A

H2A

unspecified role

Gaussia luciferase tag, N or C terminal fragment

His2A

H2A

unspecified role

Gaussia luciferase tag, N or C terminal fragment

His2A

H2A

unspecified role

Gaussia luciferase tag, N or C terminal fragment

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Jabba

interaction domain

sufficient binding region

aa 192-320

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was live Kc167 cells; proteins produced from transfected constructs.

(Stephenson et al., 2021)

External Crossreferences and Linkouts ( 0 )

References (2)

Report Sections

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