FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
EcR, Mi-2
FlyBase ID
FBig0000109459
Interaction Network
Interactions Browser links
EcR network
Mi-2 network
Reported Interactions
FBrf0235228-1.coIP.WB
Reference
Kreher et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

Mi-2

bait
EcR 

EcR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kreher et al., 2017)
FBrf0235228-2a.coIP.WB
Reference
Kreher et al., 2017Brehm, 2017.5.22
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait

FLAG tag

EcR 
Mi-2 

Mi-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
EcR 
AF2 domain
sufficient binding region
aa 432-655

coordinates refer to EcR-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from recombinant baculovirus; prey produced from recombinant baculovirus.

(Kreher et al., 2017)
FBrf0235228-3a.coIP.WB
Reference
Kreher et al., 2017Brehm, 2017.5.22
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

Mi-2

bait

FLAG tag

Mi-2 
EcR 

EcR

prey
EcR 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
EcR 
AF2 domain
sufficient binding region
aa 432-655

coordinates refer to EcR-PB

Mi-2 
ATPase domain
sufficient binding region
aa 691-1270

coordinates refer to Mi-2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from recombinant baculovirus; prey produced from recombinant baculovirus.

(Kreher et al., 2017)

Coexpression of usp interferes in the interaction.

(Kreher et al., 2017)
FBrf0235228-5a.PD.A
Reference
Kreher et al., 2017Brehm, 2017.5.22
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

Mi-2

bait

GST tag

Mi-2 
EcR 

EcR

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mi-2 
ATPase domain
sufficient binding region
aa 691-1270

coordinates refer to Mi-2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Kreher et al., 2017)
FBrf0245203-10.coIP.WB
Reference
Mazina et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / prepupal stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mi-2 

Mi-2

bait
EcR 

EcR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of wild-type prepupae; bait produced from endogenous gene; prey produced from endogenous gene.

(Mazina et al., 2020)
FBrf0245203-18.PL.MS
Reference
Mazina et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait

APEX2 tag

Mi-2 

Mi-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Mazina et al., 2020)
FBrf0245203-2.coIP.WB
Reference
Mazina et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / prepupal stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
EcR 

EcR

bait
Mi-2 

Mi-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extracts of wild-type prepupae; bait produced from endogenous gene; prey produced from endogenous gene.

(Mazina et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)