FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
orb2, ps
FlyBase ID
FBig0000110502
Interaction Network
Interactions Browser links
orb2 network
ps network
Reported Interactions
FBrf0235552-1.EMSA.A
Reference
Gill et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ps 

ps

bait

GST tag

orb2 

orb2

prey

[32]P label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Gill et al., 2017)
FBrf0235552-2.PD.WB
Reference
Gill et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
orb2 

orb2

bait

biotin label

orb2 
orb2 
orb2 
ps 

ps

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
orb2 
orb2A intron
sufficient binding region
TCCAAAATTCAAAAATCATCCACTAAAAATTGC
orb2 
orb2A intron
mutation decreasing interaction
TCCAAAATTCAAAAATAATCCACTAAAAATTGC

TCATCCAC to TAATCCAC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from S2 cell extract.

(Gill et al., 2017)
FBrf0235552-3.PD.WB
Reference
Gill et al., 2017
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
orb2 

orb2

bait

biotin label

orb2 
orb2 
orb2 
ps 

ps

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
orb2 
orb2A intron
sufficient binding region
TCCAAAATTCAAAAATCATCCACTAAAAATTGC
orb2 
orb2A intron
mutation decreasing interaction
TCCAAAATTCAAAAATAATCCACTAAAAATTGC

TCATCCAC to TAATCCAC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey derived from wild-type adult head lysate.

(Gill et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)