FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000110545
Interaction Network
Interactions Browser links
Atg8a network
dwg network
Reported Interactions
FBrf0235788-1519.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atg8a-RA

bait

fused to GAL4 DNA-binding domain

dwg 

dwg-RA

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pairwise.)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0235788-1519.Y2Hpool
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atg8a-RA

bait

fused to GAL4 DNA-binding domain

dwg 

dwg-RA

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pooled)

MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0256286-13.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atg8a

bait

fused to GAL4 DNA-binding domain

dwg 

dwg

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0256286-29.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atg8a

bait

GFP tag

dwg 

Dwg

prey

FLAG tag

dwg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dwg 
interaction domain
sufficient binding region
aa 1-150aa 385-592

coordinates relative to dwg-PA

coordinates relative to dwg-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0261033-1.Y2H
Description
physical association
Assay
Collection
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atg8a

bait

fused to LexA DNA-binding domain

dwg 

dwg

prey

fused to GAL4 activation domain

dwg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dwg 
interaction domain
sufficient binding region
aa 1-180

coordinates relative to dwg-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from larval cDNA expression library).

FBrf0261033-2.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Atg8a

bait

GST tag

dwg 

dwg

prey

His tag

dwg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
LIR motif docking site residue 49
mutation decreasing interaction
aa 49

Y49A; coordinates relative to Atg8a-PA

dwg 
LIR motif aromatic and hydrophobic residues 129 and 132
mutation decreasing interaction
aa 129 and 132

Y129A and I132A within predicted LIR motif aa 127-132; coordinates relative to dwg-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)