FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
cry, brp
FlyBase ID
FBig0000110669
Interaction Network
Interactions Browser links
cry network
brp network
Reported Interactions
FBrf0235809-1.coIP.WB
Reference
Damulewicz et al., 2017
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brp 

brp

bait
cry 

cry

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Damulewicz et al., 2017)
FBrf0235809-2.coIP.WB
Reference
Damulewicz et al., 2017
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

cry

bait

HA tag

brp 

brp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Damulewicz et al., 2017)

Interaction is stronger in light conditions.

(Damulewicz et al., 2017)
FBrf0235809-3.Y2H
Reference
Damulewicz et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

cry

bait

fused to lexA DNA-binding domain

brp 

brp

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Damulewicz et al., 2017)

Two-hybrid system: yeast lexA-BD/B42-AD. Interaction is stronger in light conditions.

(Damulewicz et al., 2017)
FBrf0235809-4.PLA
Reference
Damulewicz et al., 2017
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cry 

cry

neutral component
cry 

cry

neutral component
cry 

cry

neutral component
brp 

brp

neutral component
brp 

brp

neutral component
brp 

brp

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; proteins produced from endogenous genes.

(Damulewicz et al., 2017)

cry-brp complexes were detected in the retina, lamina and medulla in wild-type flies but not in cry mutants.

(Damulewicz et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)