FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Miro, milt
FlyBase ID
FBig0000110906
Interaction Network
Interactions Browser links
Miro network
milt network
Reported Interactions
FBrf0194133-8.coIP.WB
Reference
Glater et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Miro 

Miro

bait

T7 tag

Miro 
milt 

Milt

prey
milt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
milt 
N terminal domain with coiled coil motif
sufficient binding region
aa 1-750

coordinates relative to milt-PA

Miro 
N terminal domain
sufficient binding region
aa 1-574
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Glater et al., 2006)
FBrf0257379-1.coIP.WB
Reference
Mattedi et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Miro 

Miro

bait

GFP tag

Miro 
milt 

Milton

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Miro 
C-terminal transmembrane domain
necessary binding region
unspecified

coordinates relative to Miro-PE

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

split-Miro is a photo-cleavable construct whose EGFP tagged C-terminal domain comes together with its mCherry tagged N-terminal domain in this experiment. These modifications did not affect its ability to interact with milt.

(Mattedi et al., 2023)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Mattedi et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)