FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
lz, Mlf
FlyBase ID
FBig0000112310
Interaction Network
Interactions Browser links
lz network
Mlf network
Reported Interactions
FBrf0236362-6.coIP.WB
Reference
Miller et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
lz 

Lz

bait

GFP tag

Mlf 

MLF

prey

HA tag

Mlf 
Mlf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mlf 
MHD domain
sufficient binding region
aa 96-202aa 2-147
Mlf 
MHD domain
necessary binding region
aa 1-202
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Miller et al., 2017)
FBrf0236362-9.PD.A
Reference
Miller et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mlf 

MLF

bait

GST tag

lz 

Lz

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Miller et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)