Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.
Bait protein was fused to a plasma membrane anchor, which targets the cell membrane. The ability of this chimera to recruit prey protein to the plasma membrane was taken as evidence of direct physical interaction.
Source was cell extract of S2 cell line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct or endogenous gene.
