FlyBase Gene Report: Dmel\rin

General Information

Symbol

Dmel\rin

Species

D. melanogaster

Name

rasputin

Annotation Symbol

CG9412

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015778

Gene Model Status

Current

Stock Availability

18 publicly available

Enzyme Name (EC)

DNA helicase (3.6.4.12)

RNA helicase (3.6.4.13)

Gene Summary

rasputin (rin) encodes an RNA-binding protein that regulates gene expression. It is involved in eye development and the dorsal/ventral axis specification of the ovary. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

Nts, Next to squid, rasp

Key Links

Genomic Location

Cytogenetic map

87F6-87F7

Sequence location

3R:13,647,004..13,654,527 [+]

Recombination map

3-54

RefSeq locus

NT_033777 REGION:13647004..13654527

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:lig; FB:FBgn0020279

(Baumgartner et al., 2013)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables DNA helicase activity

inferred from sequence or structural similarity with UniProtKB:Q13283

(Gene Ontology Curators, 2002-)

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR035979

(InterPro Project Members, 2004-)

enables RNA binding

inferred from electronic annotation with InterPro:IPR000504

(InterPro Project Members, 2004-)

enables RNA helicase activity

inferred from sequence or structural similarity with UniProtKB:Q13283

(Gene Ontology Curators, 2002-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in compound eye photoreceptor fate commitment

inferred from mutant phenotype

(Pazman et al., 2000)

involved_in dorsal/ventral axis specification, ovarian follicular epithelium

inferred from mutant phenotype

(Costa et al., 2013)

inferred from genetic interaction with FLYBASE:orb; FB:FBgn0004882

(Costa et al., 2013)

involved_in ommatidial rotation

inferred from mutant phenotype

(Pazman et al., 2000)

involved_in positive regulation of gene expression

inferred from mutant phenotype

(Costa et al., 2013)

involved_in response to amino acid starvation

inferred from direct assay

(Aguilera-Gomez et al., 2017)

involved_in stress granule assembly

inferred from mutant phenotype

(Aguilera-Gomez et al., 2017)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in stress granule assembly

inferred from biological aspect of ancestor with PANTHER:PTN000074905

(GO Reference Genome Project, 2011-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

is_active_in cytoplasmic stress granule

inferred from direct assay

(Ricolo et al., 2025)

located_in cytoplasmic stress granule

inferred from direct assay

(Aguilera-Gomez et al., 2017)

located_in cytoplasm

inferred from high throughput direct assay

(Lee et al., 2014)

inferred from direct assay

(Baumgartner et al., 2013)

located_in cytosol

inferred from direct assay

(Pazman et al., 2000)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

part_of ribonucleoprotein complex

inferred from physical interaction with FLYBASE:orb; FB:FBgn0004882

(Costa et al., 2013)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasmic stress granule

inferred from biological aspect of ancestor with PANTHER:PTN000074905

(GO Reference Genome Project, 2011-)

is_active_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN000074904

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UNCLASSIFIED RNA HELICASES

UNCLASSIFIED DNA HELICASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

DNA helicase activity

ATP + H2O = ADP + phosphate + H(+) (3.6.4.12)

RNA helicase activity

ATP + H2O = ADP + phosphate + H(+) (3.6.4.13)

Summaries

Gene Snapshot

Gene Group (FlyBase)

UNCLASSIFIED RNA HELICASES -: This group comprises RNA helicases that do not classify under other groups in FlyBase.
UNCLASSIFIED DNA HELICASES -: This group comprises DNA helicases that do not classify under other groups in FlyBase.

Summary (Interactive Fly)

component of stress granules - interacts with several protein partners under both stress and non-stress conditions including Caprin, FMR1 and Lingerer - Sec16, a component of the endoplasmic reticulum exit site, is a Rasputin interactor and stabilizer - a positive regulator of orb in oogenesis - FMR1, Rasputin and Caprin act together with the UBA protein Lingerer to restrict tissue growth

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\rin for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VFT4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

3UJM

Comments on Gene Model

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

rin-RB

FBtr0082797

NM_080168

3268

690

rin-RE

FBtr0082799

NM_169532

3501

690

rin-RF

FBtr0346346

NM_169531

3167

690

rin-RG

FBtr0346347

NM_169533

5382

690

Additional Transcript Data and Comments

Reported size (kB)

3.314 (longest cDNA)

(Pazman et al., 2000)

4.2, 3.7, 3.0 (northern blot)

(Kelley, 1993)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

rin-PB

FBpp0082265

74.9

690

7.41

FBpp0082267

74.9

690

7.41

FBpp0312063

74.9

690

7.41

FBpp0312064

74.9

690

7.41

Q9VFT4

NP_731830

AAF54965

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

690 aa isoforms: rin-PB, rin-PE, rin-PF, rin-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

690 (aa); 75 (kD observed)

(Pazman et al., 2000)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

3UJM

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\rin using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

rin_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.20

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

(Pazman et al., 2000)

adult stage | female

(Pazman et al., 2000)

Additional Descriptive Data

rin transcripts are detected in embryos, larvae, early and late pupae, and adult females, with highest levels of transcripts in 0-3 hr embryos.

(Pazman et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

larval somatic muscle cell

(van der Laan et al., 2012)

adult stage | female & oogenesis

female germline cell

(Costa et al., 2013)

female germline cyst

(Costa et al., 2013)

follicle cell

(Costa et al., 2013)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

ovary

(Pisa et al., 2009)

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Pazman et al., 2000)

third instar larval stage

eye disc

(Pazman et al., 2000)

photoreceptor neuron | precursor

(Pazman et al., 2000)

Additional Descriptive Data

rin protein is distributed diffusely in the cytoplasm of embryonic muscles.

(van der Laan et al., 2012)

rin protein is detected throughout development. In developing eye imaginal discs, rin protein is localized apically in photoreceptor precursor cells, in a pattern similar to that of other Ras pathway proteins Sos, dos and drk.

(Pazman et al., 2000)

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of catalytic step 2 spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

is_active_in cytoplasmic stress granule

inferred from direct assay

(Ricolo et al., 2025)

located_in cytoplasmic stress granule

inferred from direct assay

(Aguilera-Gomez et al., 2017)

located_in cytoplasm

inferred from high throughput direct assay

(Lee et al., 2014)

inferred from direct assay

(Baumgartner et al., 2013)

located_in cytosol

inferred from direct assay

(Pazman et al., 2000)

part_of precatalytic spliceosome

inferred from high throughput direct assay

(Herold et al., 2009)

part_of ribonucleoprotein complex

inferred from physical interaction with FLYBASE:orb; FB:FBgn0004882

(Costa et al., 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\rin in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\rin

View exonic expression by tissue for Dmel\rin

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015778

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

47998/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015778

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015778

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015778

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9412

Flygut - An atlas of the Drosophila adult midgut

FBgn0015778

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 18 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of rin

Transgenic constructs containing regulatory region of rin

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(3R)Exel6169

(Ryder, 2004.4.26)

Df(3R)l26c

(Costa et al., 2013)

Df(3R)rin²

(Pazman et al., 2000)

Df(3R)urd

(Costa et al., 2013, Pazman et al., 2000)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | female limited, with Scer\GAL4^dome-PG14

rin^HMS00269

lethal | recessive

rin^LA00048

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

rin^GD14212

partially lethal - majority live (with Df(3R)urd)

partially lethal - majority live | recessive

rin³

viable

viable (with rin¹)

rin²

viable (with rin²)

viable (with rin^NP3248)

rin²

viable (with rin^NP5420)

rin²

Sterility

Allele

female sterile (with Df(3R)l26c)

rin³

female sterile (with Df(3R)urd)

rin³

female sterile | recessive

rin³

fertile

rin^EY05170

sterile

rin^unspecified

Other Phenotypes

Allele

abnormal planar polarity, with Scer\GAL4^hs.2sev

rin^UAS.cPa

abnormal planar polarity | recessive

abnormal size | adult stage | somatic clone

rin²

increased cell number | somatic clone

rin²

short lived | recessive

rin³

some die during pupal stage, with Scer\GAL4^pnr-MD237

rin^GD14212

visible

rin^unspecified

visible, with Scer\GAL4^hs.2sev

rin^UAS.cPa

visible (with rin¹)

rin²

visible (with rin²)

rin¹

visible | recessive

wild-type

rin^Tub.PP

Phenotype manifest in

Allele

actin cytoskeleton | oogenesis stage S10

rin³

actin cytoskeleton | oogenesis stage S11

rin³

chromatin

rin³

egg | maternal effect (with rin²)

rin³

egg | maternal effect (with rin³)

rin²

egg chamber

rin³

embryonic/larval cuticle | maternal effect (with rin²)

rin³

embryonic/larval cuticle | maternal effect (with rin³)

rin²

eye

eye, with Scer\GAL4^hs.2sev

rin^UAS.cPa

eye (with rin¹)

rin²

eye (with rin²)

rin¹

eye disc

eye disc, with Scer\GAL4^hs.2sev

rin^UAS.cPa

eye photoreceptor cell

eye photoreceptor cell, with Scer\GAL4^hs.2sev

rin^UAS.cPa

nucleus | increased number

rin³

nurse cell

rin³

nurse cell ring canal

rin³

ommatidial precursor cluster, with Scer\GAL4^hs.2sev

rin^UAS.cPa

ommatidium

ommatidium, with Scer\GAL4^hs.2sev

rin^UAS.cPa

ommatidium (with rin¹)

rin²

ommatidium (with rin²)

rin¹

ommatidium | somatic clone | increased number

rin²

wing

rin^unspecified

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (20)

Hsap\G3BP2

11 of 14

Yes

Hsap\G3BP1

10 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (8)

Rnor\G3bp2

12 of 14

Yes

Rnor\G3bp1

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (9)

Mmus\G3bp1

10 of 14

Yes

Mmus\G3bp2

10 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (16)

Xtro\g3bp1

9 of 13

Yes

Xtro\g3bp2

6 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\st13

1 of 13

Xtro\tram2

1 of 13

Xtro\XB5727634

1 of 13

Yes

Danio rerio (Zebrafish) (11)

Drer\g3bp2a

11 of 14

Yes

Drer\g3bp2b

11 of 14

Yes

Drer\g3bp1

10 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (14)

Cele\gtbp-1

6 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (12)

Agam\AgaP_AGAP000403

9 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (32)

Atha\AT1G13730

11 of 13

Yes

Atha\AT5G60980

11 of 13

Yes

Atha\AT2G03640

10 of 13

Yes

Atha\AT3G25150

10 of 13

Yes

Atha\AT5G48650

10 of 13

Yes

Atha\AT1G69250

9 of 13

Yes

Atha\AT5G43960

9 of 13

Yes

Atha\AT3G07250

4 of 13

Yes

Atha\AT3G24463

4 of 13

Yes

Atha\AT3G55540

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\BRE5

5 of 13

Yes

Scer\NTF2

1 of 13

Scer\PAB1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\nxt3

7 of 12

Yes

Spom\nxt1

1 of 12

Spom\nxt2

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:rin. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (7)

bol

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

sqd

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

rin^KK108430

exacerbates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\poly-GR^50.UAS.EGFP

(Lee et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

G3BP2; G3BP stress granule assembly factor 2

11 of 14

620020

G3BP1; G3BP stress granule assembly factor 1

10 of 14

608431

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

rin - Myc

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

rin - Pi3K92E

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

rin - Rheb

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

rin - Sec16

anti bait coimmunoprecipitation, western blot, phenotype-based detection assay, fluorescence microscopy, inferred by author, affinity technology, Identification by mass spectrometry

(Aguilera-Gomez et al., 2017)

rin - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

rin - Zrf1

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Shun Li et al., 2025)

rin - cbc

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Wu et al., 2021)

rin - csw

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

rin - dos

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

rin - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

rin - heca

proximity ligation assay, fluorescence microscopy

(Ricolo et al., 2025)

rin - lig

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, two hybrid, peptide massfingerprinting, coimmunoprecipitation

(Dong et al., 2015, Kimura et al., 2015, Baumgartner et al., 2013)

rin - orb

anti bait coimmunoprecipitation, western blot, peptide massfingerprinting

(Costa et al., 2013, Costa et al., 2005)

rin - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

rin - sina

two hybrid

(Hu et al., 2017.6.13)

RNA-protein

Physical Interaction

Assay

References

rin - Mic10b

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Laver et al., 2020)

rin - ND-AGGG

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Laver et al., 2020)

rin - Nop10

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Laver et al., 2020)

rin - Phf5a

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Laver et al., 2020)

rin - gish

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Laver et al., 2020)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

orb

enhanceable

rin

(Costa et al., 2013)

Ras85D

suppressible

rin

(Pazman et al., 2000)

Rho1

enhanceable

rin

(Pazman et al., 2000)

sev

suppressible

rin

(Pazman et al., 2000)

sev|Sos

enhanceable

rin

(Pazman et al., 2000)

svp

suppressible

rin

(Pazman et al., 2000)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

71089

DroID - A comprehensive database of gene and protein interactions.

FBgn0015778

MIST (genetic) - An integrated Molecular Interaction Database

47998

MIST (protein-protein) - An integrated Molecular Interaction Database

47998

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-54

Cytogenetic map

87F6-87F7

Sequence location

3R:13,647,004..13,654,527 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87F6-87F7

Limits computationally determined from genome sequence between P{PZ}CtBP⁰³⁴⁶³ and P{lacW}B52^s2249&P{lacW}flfl^L4179

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

87F-87F

(Pazman, 2000.2.2)

87F3-87F6

(determined by in situ hybridisation)

(Pazman et al., 2000)

87F4-87F10

(Kelley, 1993)

Experimentally Determined Recombination Data

Location

3-54

(FlyBase, 2016)

3-51.0

(Comeron et al., 2012)

(Kelley, 1993)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (18)

Aberrations including deletions of this gene Aberrations including duplications of this gene

9301

9304

9309

9302

92837

602389

y¹ w¹; P{TKO-UAS.GS06196}attP40/CyO

19850

y¹ w^67c23; P{EPgy2}rin^EY05170

57694

w^*; rin³/TM6B, Sb¹ Tb¹

FlyORF

F001812

M{UAS-rin.ORF}ZH-86Fb

Kyoto

104425

w^*; P{GawB}rin^NP3248

VDRC

v109911

P{KK108430}VIE-260B

v29113

w¹¹¹⁸ P{GD14212}v29113

More stocks available...

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (254)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015778

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015778

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Laver et al., 2020)

polyclonal

(Aguilera-Gomez et al., 2017, Pazman et al., 2000)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

rin is required as an effector in Ras signaling during eye development.

(Pazman et al., 2000)

Mutants show defects in photoreceptor recruitment and ommatidial polarity in the eye.

(Pazman et al., 2000)

rin is required for the normal development of egg chambers.

(Haynes and Pazman, 1999)

Egg chambers of homozygous mutant females show variable defects, including abnormal numbers of nurse cells, premature degeneration of nurse cell nuclei, small oocytes and abnormal distribution of follicle cells.

(Haynes and Pazman, 1999)

Transcription unit defined during a molecular analysis of sqd.

(Kelley, 1993)

Relationship to Other Genes

Source for database merge of

Source for merge of: rin CG9412

(FlyBase, 1996-)

Additional comments

Source for merge of rin CG9412 was sequence comparison ( date:000515 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG9412

(Kolkhof et al., 2017, Çiçek et al., 2016, Brooks et al., 2015, Horan et al., 2015, Rosa-Ferreira et al., 2015, Schertel et al., 2013, Yoshigi et al., 2013, Ni et al., 2010.12.1, Herold et al., 2009, Keleman et al., 2009.8.5, Pisa et al., 2009, Quinones-Coello, 2007, Cermelli et al., 2006, Duncan et al., 2006)

Nts

(Burnette et al., 1999, Haynes et al., 1997, Kelley, 1993)

RIN

(Anderson et al., 2018, van der Laan et al., 2012, Sofola et al., 2007)

Rin

(Milano et al., 2025, Ricolo et al., 2025, Pushpalatha et al., 2022, Sadasivan et al., 2022, Singh et al., 2022, Aguilera-Gomez et al., 2017, Khong et al., 2017, Dong et al., 2015, Kimura et al., 2015, Lee et al., 2014, Khong and Jan, 2011, Rasmussen and Kellis, 2007)

cg9412

(Lasko, 2000)

rasp

(Pazman and Haynes, 1999, Pazman and Haynes, 1998)

Name Synonyms

Next to squid

(Haynes et al., 1997, Kelley, 1993)

Rasputin

(Anderson et al., 2018, Khong et al., 2017, Kimura et al., 2015, Costa et al., 2013, Vognsen and Kristensen, 2012, Beerman and Jongens, 2011, Khong and Jan, 2011, Pisa et al., 2009, Chang et al., 2008)

rasputin

(Dyson et al., 2025, Cermelli et al., 2006, Pazman, 2000.2.2, Pazman et al., 2000, Haynes and Pazman, 1999)

Secondary FlyBase IDs

FBgn0038164

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 66 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

47998

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VFT4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VFT4

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015778

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

47998/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015778

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015778

FlyMine - An integrated database for Drosophila genomics

FBgn0015778

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG9412

MARRVEL_MODEL - MARRVEL (model organism gene)

47998

PDB - An information portal to biological macromolecular structures

3UJM

Linkouts

BioGRID - A database of protein and genetic interactions.

71089

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015778

DroID - A comprehensive database of gene and protein interactions.

FBgn0015778

DRSC - Results frm RNAi screens

FBgn0015778

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

rin_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015778

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015778

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9412

Flygut - An atlas of the Drosophila adult midgut

FBgn0015778

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC000929

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

47998

MIST (protein-protein) - An integrated Molecular Interaction Database

47998

References (122)

The following external sites may use different assemblies or annotations than FlyBase.

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