lig, rin, Capr and Fmr1 also co-localize in cytoplasmic punctae upon overexpression.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

lig interacts with rin, but not Capr or Fmr1 in the yeast two hybrid assay.

The reciprocal interaction of lin with rin, in which the activation and DNA-binding domains were switched, was not observed.

The interaction is RNA-independent.

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.