FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Mlf, DnaJ-1
FlyBase ID
FBig0000113079
Interaction Network
Interactions Browser links
Mlf network
DnaJ-1 network
Reported Interactions
FBrf0236075-1.TAP.MP
Reference
Dyer et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Mlf 

MLF

bait

HA, FLAG tag

DnaJ-1 

DnaJ-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Dyer et al., 2017)
FBrf0236075-10.coIP.MW
Reference
Dyer et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mlf 

MLF

bait

His tag

DnaJ-1 

DnaJ-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Dyer et al., 2017)
FBrf0236075-12.CH.WB
Reference
Dyer et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Mlf 

MLF

bait

HA, FLAG tag

DnaJ-1 

DnaJ-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Dyer et al., 2017)
FBrf0236075-4.TAP.MP
Reference
Dyer et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
DnaJ-1 

DnaJ-1

bait

HA, FLAG tag

Mlf 

MLF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Dyer et al., 2017)
FBrf0236362-1.coIP.MS
Reference
Miller et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Mlf 

MLF

bait

V5 tag

DnaJ-1 

DnaJ-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Miller et al., 2017)
FBrf0236362-2.coIP.WB
Reference
Miller et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Mlf 

MLF

bait

GFP tag

DnaJ-1 

DnaJ-1

prey

HA tag

DnaJ-1 
DnaJ-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
DnaJ-1 
C-terminal domain
sufficient binding region
aa 157-334aa 2-191
DnaJ-1 
C-terminal domain
necessary binding region
aa 157-334
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Miller et al., 2017)
FBrf0236362-3.coIP.WB
Reference
Miller et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
DnaJ-1 

DnaJ-1

bait

GFP or HA tag

DnaJ-1 
Mlf 

MLF

prey

HA or GFP tag

Mlf 
Mlf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mlf 
MHD domain
sufficient binding region
aa 96-202
Mlf 
MHD domain
necessary binding region
aa 1-202
DnaJ-1 
C-terminal domain
sufficient binding region
aa 157-334
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Miller et al., 2017)
FBrf0236362-4.PD.A
Reference
Miller et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
DnaJ-1 

DnaJ-1

bait

GST tag

Mlf 

MLF

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Miller et al., 2017)
FBrf0236362-5.PD.A
Reference
Miller et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mlf 

MLF

bait

GST tag

DnaJ-1 

DnaJ-1

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Miller et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)