FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ppa, cid
FlyBase ID
FBig0000113228
Interaction Network
Interactions Browser links
Ppa network
cid network
Reported Interactions
FBrf0215570-1.Y2H
Reference
Moreno-Moreno et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cid 

CenH3

bait

fused to LexA DNA-binding domain

cid 
Ppa 

Ppa

prey

fused to B42 activation domain

Ppa 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cid 
interaction domain
sufficient binding region
aa 1-123
Ppa 
C-terminal 92 residues
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Moreno-Moreno et al., 2011)

Two-hybrid system: yeast LexA-BD/B42-AD

(Moreno-Moreno et al., 2011)
FBrf0215570-2.PD.A
Reference
Moreno-Moreno et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cid 

CenH3

bait

GST tag

cid 
Ppa 

Ppa

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cid 
interaction domain
sufficient binding region
aa 1-123aa 124-225
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Moreno-Moreno et al., 2011)

Ppa bound more stongly to cid fragment 124-225 than 1-123.

(Moreno-Moreno et al., 2011)
FBrf0215570-3.PD.A
Reference
Moreno-Moreno et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ppa 

Ppa

bait

GST tag

cid 

CenH3

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Moreno-Moreno et al., 2011)
FBrf0215570-4.PD.WB
Reference
Moreno-Moreno et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ppa 

Ppa

bait

GST tag

cid 

CenH3 Ppa

prey

TAP tag

cid 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cid 
interaction domain
necessary binding region
aa 161-200
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell nucleosome preparation.

(Moreno-Moreno et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)