FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rho1, slpr
FlyBase ID
FBig0000113235
Interaction Network
Interactions Browser links
Rho1 network
slpr network
Reported Interactions
FBrf0210554-1.coIP.WB
Reference
Neisch et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho1

bait

FLAG tag

Rho1 
slpr 

Slpr

prey
slpr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rho1 
interaction domain
mutation increasing interaction
aa 19

N19

slpr 
kinase domain
mutation decreasing interaction
aa 141-445

deletion of aa 141-445

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene or transfected construct.

(Neisch et al., 2010)
FBrf0210554-4.PD.A
Reference
Neisch et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rho1 

Rho1

bait

GST tag

slpr 

Slpr

prey

[35]S label

slpr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
slpr 
N-terminal domain
sufficient binding region
aa 14-540
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Neisch et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)