Edc3, me31B Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

me31B

Me31b

bait

HA tag

Edc3

EDC3

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Tritschler et al., 2007)

FBrf0203000-5.coIP.WB

Reference

Tritschler et al., 2007

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Edc3

EDC3

bait

HA tag

Edc3

me31B

Me31b

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Edc3

C-terminal domain

sufficient binding region

aa 331-680

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tritschler et al., 2007)

FBrf0206975-4.coIP.WB

Reference

Jinek et al., 2008

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

me31B

Me31B

bait

HA tag

Edc3

EDC3

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jinek et al., 2008)

Positive control.

(Jinek et al., 2008)

FBrf0206982-11.coIP.WB

Reference

Tritschler et al., 2008

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Edc3

EDC3

bait

HA tag

me31B

Me31B

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tritschler et al., 2008)

FBrf0206982-5.coIP.WB

Reference

Tritschler et al., 2008

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

me31B

Me31B

bait

HA tag

me31B

Edc3

EDC3

prey

GFP tag

Edc3

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

me31B

RecA domain

sufficient binding region

aa 268-459

coordinates relative to me31B-PA

Edc3

N-terminal domain

sufficient binding region

aa 1-440

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene or transfected construct.

(Tritschler et al., 2008)

FBrf0210604-10.coIP.WB

Reference

Haas et al., 2010

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Edc3

EDC3

bait

HA tag

me31B

Me31B

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Haas et al., 2010)

FBrf0210604-9.coIP.WB

Reference

Haas et al., 2010

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

me31B

Me31B

bait

HA tag

me31B

Edc3

EDC3

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

me31B

interaction domain

mutation disrupting interaction

aa 281,284,288,292

Q281A,H284A,Y288A,K292A, coordinates relative to me31B-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Haas et al., 2010)

FBrf0215484-1.coIP.WB

Reference

Tritschler et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

me31B

Me31B

bait

HA tag

me31B

Edc3

DmEDC3

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

me31B

interaction domain residues 281,284

mutation disrupting interaction

aa 281,284

Q281A,H284A coordinates relative to me31B-PA

me31B

interaction domain residues 288,292

mutation disrupting interaction

aa 288,292

T288A,K292A coordinates relative to me31B-PA

me31B

interaction domain residues 405,408

mutation disrupting interaction

aa 405,408

F405A,H408A coordinates relative to me31B-PA

me31B

interaction domain residues 411,412

mutation disrupting interaction

aa 411,412

E411A,K412A coordinates relative to me31B-PA

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene or transfected construct.

(Tritschler et al., 2009)

FBrf0215484-3.coIP.WB

Reference

Tritschler et al., 2009

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Edc3

DmEDC3

bait

HA tag

Edc3

me31B

Me31B

prey

GFP tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Edc3

interaction domain residue 345

mutation decreasing interaction

aa 345

F345A

Edc3

interaction domain residue 346

mutation decreasing interaction

aa 346

D346A

Edc3

interaction domain residue 344,346

mutation decreasing interaction

aa 344,346

D344K,D346K

Edc3

interaction domain residue 354

mutation disrupting interaction

aa 354

F354A

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tritschler et al., 2009)

FBrf0236770-25.coIP.WB

Reference

Wang et al., 2017

Description

physical association

Assay

Collection

Source/Stage

Cell line used

S2-ThermoFischer

Participants

Corresponds to

Reported as

Role

Note

me31B

ME31B

bait

GFP tag

Edc3

EDC3

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Wang et al., 2017)

FBrf0244109-7.coIP.WB

Reference

Peter et al., 2019

Description

physical association

Assay

Collection

Source/Stage

Cell line used