FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
tio, CtBP
FlyBase ID
FBig0000113726
Interaction Network
Interactions Browser links
tio network
CtBP network
Reported Interactions
FBrf0216557-3.Y2H
Reference
Datta et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tio 

Tio

bait

fused to GAL4 DNA-binding domain

CtBP 

CtBP

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from eye-antennal imaginal disc enriched larval cDNA expression library).

(Datta et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Datta et al., 2011)
FBrf0216557-5.coIP.WB
Reference
Datta et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
tio 

TIO

bait

Myc tag

CtBP 

CTBP

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of Kc167 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Datta et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)