FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000115719
Interaction Network
Interactions Browser links
csw network
stumps network
Reported Interactions
FBrf0174966-3.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
csw 

Csw

bait

Dof

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N terminal domain
sufficient binding region
aa 1-600

coordinates relative to stumps-PA

Tyrosine 515
mutation decreasing interaction
aa 515

Y515F, coordinates relative to stumps-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Substitution of the tyrosine 515 with phenylalanine significantly weakened the formation of a csw/stumps complex upon btl stimulation, demonstrating that tyrosine 515 of stumps plays an important role in the recruitment of csw. Mutation of tyrosine 486 had no effect on the recruitment of csw.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)