FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000115766
Interaction Network
Interactions Browser links
Polr2A network
pasha network
Reported Interactions
FBrf0236708-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Pol II

bait

Pasha

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

Treatment of S2 cells with flavopiridol, a Cdk9 inhibitor, reduces the RpII215-pasha interaction.

FBrf0236708-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Pasha

bait

GFP tag

Pol II

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

Both S2P- and SP5-modified forms of RpII215 are detected in pasha immunoprecipitate.

FBrf0236708-3.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Pasha

bait

GST tag

Pol II

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
WWD domain
sufficient binding region
aa 153-206

coordinates relative to pasha-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell nuclear extract.

S2P-modified form of RpII215 is detected in GST-pasha pull-down. Treatment of S2 cells with flavopiridol, a Cdk9 inhibitor, reduces the RpII215-pasha interaction.

FBrf0236708-4.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Pol II

bait

Pasha

prey

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
WW domain
sufficient binding region
aa 153-185

coordinates relative to pasha-PA

CTD heptad domain
sufficient binding region
YSPTSPSYSPTSPSYSPTSPSYSPTSPS
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait peptides synthesized in vitro; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)